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icecold dpbs  (Mediatech)

 
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    Structured Review

    Mediatech icecold dpbs
    Icecold Dpbs, supplied by Mediatech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/icecold+dpbs/icecold+dpbs/pm23440329-54-15-21
    Average 90 stars, based on 1 article reviews
    icecold dpbs - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: PRAF2 stimulates cell proliferation and migration and predicts poor prognosis in neuroblastoma.
    Article Snippet: .. For total protein isolation, medium was removed by aspiration, adherent cells were washed twice in icecold DPBS (Dulbecco's phosphate buffered saline, Mediatech) and cell lysates prepared by scraping on ice into radioimmunoprecipitation assay (RIPA) buffer [20 mM Tris-HCl (pH 7.5), 0.1% (w/v) sodium lauryl sulfate, 0.5% (w/v) sodium deoxycholate, 135 mM NaCl, 1% (v/v) Triton X-100, 10% (v/v) glycerol, 2 mM EDTA], supplemented with Complete Protease Inhibitor Cocktail (Roche Diagnostic Corp., Indianapolis, IN) and phosphatase inhibitors (20 mM sodium fluoride and 0.27 mM sodium vanadate). ..

    Saline:

    Article Title: PRAF2 stimulates cell proliferation and migration and predicts poor prognosis in neuroblastoma.
    Article Snippet: .. For total protein isolation, medium was removed by aspiration, adherent cells were washed twice in icecold DPBS (Dulbecco's phosphate buffered saline, Mediatech) and cell lysates prepared by scraping on ice into radioimmunoprecipitation assay (RIPA) buffer [20 mM Tris-HCl (pH 7.5), 0.1% (w/v) sodium lauryl sulfate, 0.5% (w/v) sodium deoxycholate, 135 mM NaCl, 1% (v/v) Triton X-100, 10% (v/v) glycerol, 2 mM EDTA], supplemented with Complete Protease Inhibitor Cocktail (Roche Diagnostic Corp., Indianapolis, IN) and phosphatase inhibitors (20 mM sodium fluoride and 0.27 mM sodium vanadate). ..

    Radio Immunoprecipitation:

    Article Title: PRAF2 stimulates cell proliferation and migration and predicts poor prognosis in neuroblastoma.
    Article Snippet: .. For total protein isolation, medium was removed by aspiration, adherent cells were washed twice in icecold DPBS (Dulbecco's phosphate buffered saline, Mediatech) and cell lysates prepared by scraping on ice into radioimmunoprecipitation assay (RIPA) buffer [20 mM Tris-HCl (pH 7.5), 0.1% (w/v) sodium lauryl sulfate, 0.5% (w/v) sodium deoxycholate, 135 mM NaCl, 1% (v/v) Triton X-100, 10% (v/v) glycerol, 2 mM EDTA], supplemented with Complete Protease Inhibitor Cocktail (Roche Diagnostic Corp., Indianapolis, IN) and phosphatase inhibitors (20 mM sodium fluoride and 0.27 mM sodium vanadate). ..

    Protease Inhibitor:

    Article Title: PRAF2 stimulates cell proliferation and migration and predicts poor prognosis in neuroblastoma.
    Article Snippet: .. For total protein isolation, medium was removed by aspiration, adherent cells were washed twice in icecold DPBS (Dulbecco's phosphate buffered saline, Mediatech) and cell lysates prepared by scraping on ice into radioimmunoprecipitation assay (RIPA) buffer [20 mM Tris-HCl (pH 7.5), 0.1% (w/v) sodium lauryl sulfate, 0.5% (w/v) sodium deoxycholate, 135 mM NaCl, 1% (v/v) Triton X-100, 10% (v/v) glycerol, 2 mM EDTA], supplemented with Complete Protease Inhibitor Cocktail (Roche Diagnostic Corp., Indianapolis, IN) and phosphatase inhibitors (20 mM sodium fluoride and 0.27 mM sodium vanadate). ..



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